Research: SIQUEIRA and COLLEAGUES,

Listed in Issue 205

Abstract


SIQUEIRA and COLLEAGUES, Departamento de Farmacologia, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. ionara@ufrgs.br  investigated ascorbate uptake in hippocampal slices from old Wistar rats, as well as its neuroprotective effects in in vitro and in vivo assays.

Background

Ascorbate, an intracellular antioxidant, has been considered critical for neuronal protection against oxidant stress, which is supported especially by in vitro studies. Besides, it has been demonstrated an age-related decrease in brain ascorbate levels.

Methodology

Hippocampal slices from male Wistar rats aged 4, 11 and 24 months were incubated with radiolabelled ascorbate and incorporated radioactivity was measured. Hippocampal slices from rats were incubated with different concentrations of ascorbate and submitted to H(2)O(2)-induced injury, cellular damage and S100B protein levels were evaluated. The effect of chronic administration of ascorbate on cellular oxidative state and astrocyte biochemical parameters in the hippocampus from 18-months-old Wistar rats was also studied.

Results

The ascorbate uptake was decreased in hippocampal slices from old-aged rats, while supplementation with ascorbate (2 weeks) did not modify any tested oxidative status in the hippocampus and the incubation was unable to protect hippocampal slices submitted to oxidative damage (H(2)O(2)) from old rats.

Conclusion

Our data suggest that the decline of ascorbate uptake might be involved in the brain greater susceptibility to oxidative damage with advancing age and both in vitro and vivo assays suggest that ascorbate supplementation did not protect hippocampal cells.

References

Siqueira IR, Elsner VR, Leite MC, Vanzella C, Moyses Fdos S, Spindler C, Godinho G, Battu C, Wofchuk S, Souza DO, Goncalves CA and Netto CA. Ascorbate uptake is decreased in the hippocampus of ageing rats. Source Neurochemistry International. 58(4): 527-32. Mar 2011.

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