Positive Health Online

Abstract

SABOORI and COLLEAGUES, 1. Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, HojatDoost St, Tehran, Iran; Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; 2. Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, HojatDoost St, Tehran, Iran.

3. Department of Cardiology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran; 4. Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; 5. Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; 6. Department of Basic sciences and Nutrition, Cardiovascular Research Center, School of public Health, Mazandaran University of Medical Sciences, Sari, Iran; 7. Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, HojatDoost St, Tehran, Iran. Electronic address: mjalali87@yahoo.com conducted a randomized controlled trial to assess the effects of administering omega-3 and vitamin E supplements on SIRT1 and PGC1α gene expression and serum levels of antioxidant enzymes in coronary artery disease (CAD) patients.

Background

SIRT1 and PGC1α are two important genes, which play critical roles in regulating oxidative stress and inflammation processes. The study aimed assess the effects of co-administration of omega-3 and vitamin E supplements on SIRT1 and PGC1α gene expression and serum levels of antioxidant enzymes in coronary artery disease (CAD) patients.

Methodology

Results

Participants of this randomized controlled trial included 60 CAD male patients who were categorized into three groups: Group 1 received omega-3 (4 g/day) and vitamin E placebo (OP), group 2 omega-3 (4 g/day) and vitamin E (400 IU/day; OE), and group 3 omega-3 and vitamin E placebos (PP) for 2 months. Gene expression of SIRT1 and PGC1α in peripheral blood mononuclear cells (PBMCS) was assessed by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, serum antioxidant enzyme and high-sensitivity C-reactive protein (hsCRP) levels were assessed at the beginning and end of the intervention. Gene expression of SIRT1 and PGC1α increased significantly in the OE group (P = 0.039 and P = 0.050, respectively). Catalase and hsCRP levels increased significantly in the OE and OP groups. However, glutathione peroxidase (GPX) and superoxide dismutase (SOD) levels did not statistically change in all groups. The total antioxidant capacity (TAC) increased significantly in the OE group (P = 0.009) but not in OP and PP groups.

Conclusion

Supplementation of omega-3 fatty acids in combination with vitamin E may have beneficial effects on CAD patients by increasing gene expression of SIRT1 and PGC1α and improving oxidative stress and inflammation in these patients.

References

Saboori S1, Koohdani F2, Nematipour E3, Yousefi Rad E4, Saboor-Yaraghi AA2, Javanbakht MH2, Eshraghian MR5, Ramezani A6, Djalali M7. Beneficial effects of omega-3 and vitamin E co-administration on gene expression of SIRT1 and PGC1α and serum antioxidant enzymes in patients with coronary artery disease. Nutr Metab Cardiovasc Dis. 26(6):489-94. Jun 2016. doi: 10.1016/j.numecd.2015.11.013. Epub Dec 15 2015.