Positive Health Online

Abstract

IQBAL and COLLEAGUES,  (1)Faculty of Pharmaceutical and Allied Health Sciences, Department of Pharmacology, Institute of Pharmacy, Lahore College for Women University, Lahore, Punjab, Pakistan; (2)Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Bahauddin Zakariya University, Multan, Punjab, Pakistan; (3)Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore, Punjab, Pakistan; (4)Faculty

Background

In the current study we investigated the impact of combination of rutin and vitamin A on glycated products, the glyoxalase system, oxidative markers, and inflammation in animals fed a high-fat high-fructose (HFFD) diet.

Methodology

Thirty rats were randomly divided into six groups (n = 5). The treatments, metformin (120 mg/kg), rutin (100 mg/kg), vitamin A (43 IU/kg), and a combination of rutin (100 mg/kg) and vitamin A (43 IU/kg) were given to relevant groups of rats along with high-fructose high-fat diet for 42 days. HbA1c, D-lactate, Glyoxylase-1, Hexokinase 2, malondialdehyde (MDA), glutathione peroxidase (GPx), catalase (CAT), nuclear transcription factor-B (NF-κB), interleukin-6 (IL-6), interleukin-8 (IL-8) and histological examinations were performed after 42 days. The docking simulations were conducted using Auto Dock package.

Results

RESULTS: The combined effects of rutin and vitamin A in treated rats significantly (p < 0.001) reduced HbA1c, hexokinase 2, and D-lactate levels while preventing cellular damage. The combination dramatically (p < 0.001) decreased MDA, CAT, and GPx in treated rats and decreased the expression of inflammatory cytokines such as IL-6 andIL-8, as well as the transcription factor NF-κB. The molecular docking investigations revealed that rutin had a strong affinity for several important biomolecules, including as NF-κB, Catalase, MDA, IL-6, hexokinase 2, and GPx.

Conclusion

The results propose beneficial impact of rutin and vitamin A as a convincing treatment strategy to treat Age-related disorders, such as diabetes, autism, alzheimer's, atherosclerosis. Copyright: © 2024 Iqbal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Conflict of interest statement: The authors have declared that no competing interests exist.

References

Iqbal A(1), Hafeez Kamran S(1), Siddique F(2), Ishtiaq S(3), Hameed M(4), Manzoor M(4). Modulatory effects of rutin and vitamin A on hyperglycemia induced glycation, oxidative stress and inflammation in high-fat-fructose diet animal model.  PLoS One. 2024 May 9;19(5):e0303060. doi: 10.1371/journal.pone.0303060. eCollection 2024.