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Research: GUPTA and PRAKASH
Listed in Issue 175
Abstract
GUPTA and PRAKASH, Department of Studies in Food Science & Nutrition, University of Mysore, Manasagangotri, Mysore, 570 006, India. sheetalgupta.75@gmail.com studied the antioxidant activity of various green leafy vegetables (GLV).
Background
To identify the potential of green leafy vegetables (GLV) as antioxidants, methanolic extracts of Amaranthus sp., Centella asiatica, Murraya koenigii and Trigonella foenum graecum were studied for their antioxidant activity in different systems at multiple concentrations.
Methodology
Total antioxidant activity assessed by phosphomolybdenum method, free radical scavenging activity by 1,1-diphenly-2-picryl hydrazyl (DPPH), reducing power and ferrous ion chelating activity were determined. The GLV were analyzed for ascorbic acid, total and beta-carotene and total polyphenol contents.
Results
The ascorbic acid, total carotene, beta-carotene and total phenolic content (tannic acid equivalents) of the GLV ranged between 15.18-101.36, 34.78-64.51, 4.23-8.84 and 150.0-387.50 mg/100 g GLV, respectively. The extracts were found to have significantly different levels of antioxidant activities in the systems tested. The total antioxidant activity was highest in Murraya koenigii (2,691.78 micromol of ascorbic acid/g sample) and least in Centella asiatica (623.78 micromol of ascorbic acid/g sample). The extract concentration causing 50% inhibition of DPPH (IC50) was determined (M. koenigii
Conclusion
Multiple regression analysis showed that the relationship of total antioxidant activity, free radical scavenging activity, and reducing power with polyphenol and total and beta-carotene was highly significant.
References
Gupta S and Prakash J. Studies on Indian green leafy vegetables for their antioxidant activity. Plant Foods for Human Nutrition. 64(1): 39-45. Mar 2009.
