Research: CHAPPLE and COLLEAGUES,

Listed in Issue 256

Abstract

CHAPPLE and COLLEAGUES, 1. School of Dentistry, Institute of Clinical Sciences, Collegeof Medical and Dental Sciences, University of Birmingham, 5CHAPPLE and COLLEAGUES, Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK. i.l.c.chapple@bham.ac.uk; 2. School of Dentisty, Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK; 3. School of Dentistry, Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK investigate the impact of micronutrients and macronutrients upon inflammatory pathways and biomarkers of oxidative stress.

Background

Chronic inflammatory diseases are the major causes of mortality in humans and recent research has improved our understanding of the major impact of life-style factors upon inflammatory diseases and conditions.

Methodology

One of the most influential of these is nutrition, which may drive both pro-inflammatory as well as anti-inflammatory cascades at molecular and cellular levels.

Results

There are a variety of model systems that may be employed to investigate the impact of micronutrients and macronutrients upon inflammatory pathways, many of which operate through oxidative stress, either at the level of controlling the redox state of the cell and downstream redox-regulated gene transcription factors, and other acting as free radical generating or scavenging agents.

Conclusion

This chapter focuses upon biological sample preparation prior to assay and details methods for analyzing certain antioxidant micronutrients and biomarkers of oxidative stress.

References

Chapple IL1, Griffiths HR2, Msilward MR3, Ling MR3, Grant MM3. Antioxidant Micronutrients and Oxidative Stress Biomarkers. Methods Mol Biol.1537:61-77. 2017.

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