Research: WU and COLLEAGUES,

Listed in Issue 232

Abstract

WU and COLLEAGUES,  (1)Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China; Department of Orthopedics Surgery, The First Affiliated Hospital,  College of Medicine of Xi'an Jiaotong University, Xi'an 710061, China.

(2)Department of Orthopedics Surgery, The Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.

(3)Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.

(4)Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland.

(5)Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, 7703 Floyd Curl, San Antonio, TX 78229, USA.

(6)Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China. Electronic address: Guox@mail.xjtu.edu.cn analyzed the expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck (KBD) osteoarthritis

Background

Kashin-Beck disease (KBD) is a special type of endemic osteoarthritis. It has been suggested that alterations in selenium metabolism and apoptosis play a role in KBD. However, the underlying molecular mechanism remains largely unclear.

Methodology

The authors performed a microarray analysis using RNA isolated from cartilages of KBD patients and healthy controls, through Significance Analysis of Microarray (SAM) software. Functional gene networks and crucial molecules associated with differentially expressed genes were investigated via Ingenuity Pathway Analysis (IPA) and hub gene analysis. Quantitative real-time PCR was used to check the validation of chip test.

Results

The authors identified 52 up-regulated apoptosis-related genes and 26 down-regulated selenium-related genes between KBD and controls; these genes were associated with the "MYC-mediated apoptosis signalling pathway". We confirmed the results from array studies with quantitative real-time PCR analysis.

Conclusion

The results suggest that abnormal regulation of selenium metabolism and apoptosis through the MYC mediated signalling pathway contributes to the pathogenesis of KBD, but the relationship between apoptosis gene and selenium gene was not found.

References

Wu SX(1), Wang WZ(2), Zhang F(3), Wu CY(3), Dennis BS(3), Qu CJ(4), Bai YD(5), Guo X(6). Expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck osteoarthritis. Gene. Feb 10 2014. 535(2):124-30. doi: 10.1016/j.gene.2013.11.050. Epub Dec 4 2013.

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