Research: TERASHIMA and COLLEAGUES,

Listed in Issue 241

Abstract

TERASHIMA and COLLEAGUES,  (1)Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036-8564, Japan studied the effect of Ascorbic acid (AsA) treatment combined with X-irradiation and the role of reactive oxygen species (ROS) in HL-60 human leukemia cells.

Background

Ascorbic acid (AsA) treatment is expected to be a potential cancer therapy strategy with few side-effects that can be used alone or in combination with chemotherapy. However, the combination of AsA, a free radical scavenger, with radiation is not clearly understood; conflicting data are reported for cancer cell death.

Methodology

The authors conducted this study to determine the effect of AsA treatment combined with X-irradiation and the role of reactive oxygen species (ROS) in HL-60 human promyelocytic leukemia cells.

Results

Additive cytotoxic effects were observed when the cells were exposed to 2 Gy X-irradiation after 2.5 mM AsA treatment. When catalase was added to the culture with AsA alone, the cytotoxic effects of AsA disappeared. X-irradiation increased intercellular ROS levels and mitochondrial superoxide levels. By contrast, AsA alone and in combination with X-irradiation decreased ROS levels. However, in the presence of catalase neutralizing H2O2, AsA alone or in combination with X-irradiation only slightly decreased the intercellular ROS. Moreover, AsA decreased the mitochondrial membrane potential, which is commonly associated with apoptosis. These results suggest that the reduction of ROS did not result from ROS scavenging by AsA, and AsA induced apoptosis through a ROS-independent pathway.

Conclusion

This study reports that a combination of AsA with radiation treatment is effective in cancer therapy when considering ROS in cancer cells.

References

Terashima S(1), Hosokawa Y, Yoshino H, Yamaguchi M, Nakamura T. Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: the kinetics of reactive oxygen species. Oncol Rep. 30(6):2653-8. Dec 2013. doi: 10.3892/or.2013.2758. Epub Oct 1 2013.

Comment

The above research demonstrated that a combination of Ascorbic acid (AsA) with radiation treatment is effective in cancer therapy when considering reactive oxygen species (ROS) in HL-60 human leukemia cells.

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