Positive Health Online

Abstract

PRISCILLA and COLLEAGUES,  (1)Department of Life Science, School of Life Sciences, Central University of Karnataka, Kalaburagi, Karnataka, India; (2)International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India; (3)Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, India; (4)Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India; (5)Department of Life Science, School of Life Sciences, Central University of Karnataka, Kalaburagi, Karnataka, India. rakeshkumar@cuk.ac.in .describes carotenoid extraction methods from Plant Tissues.

Background

Carotenoids are the natural pigments available in nature and exhibit different colours such as yellow, red, and orange. These are a class of phytonutrients that have anti-cancer, anti-inflammatory, anti-oxidant, immune-modulatory, and anti-aging properties. These were used in food, pharmaceutical, nutraceutical, and cosmetic industries. They are divided into two classes: carotenes and xanthophylls.

Methodology

The carotenes are non-oxygenated derivatives and xanthophylls are oxygenated derivatives. The major source of carotenoids are vegetables, fruits, and tissues. Carotenoids also perform the roles of photoprotection and photosynthesis. In addition to the roles mentioned above, they are also involved and act as precursor molecules for the biosynthesis of phytohormones such as strigolactone and abscisic acid.

Results

This chapter briefly introduces carotenoids and their extraction method from plant tissue. Proposed protocol describes the extraction of carotenoid using solvents chloroform and dichloromethane. Reverse-phase HPLC can be performed with C30 columns using gradient elution. The column C30 is preferred to the C18 column because the C30 column has salient features, which include selective nature in the separation of structural isomers and hydrophobic, long-chain compounds, and shows the best compatibility with highly aqueous mobile phases.

Conclusion

A complete pipeline for the extraction of carotenoids from plant tissue is given in the present protocol. © 2024. The Author(s), under exclusive license to Springer Science+Business  Media, LLC, part of Springer Nature.

References

Priscilla K(1), Sharma V(2)(3), Gautam A(1), Gupta P(4), Dagar R(1), Kishore

V(1), Kumar R(5). Carotenoid Extraction from Plant Tissues.  Methods Mol Biol.;2788:3-18. doi: 10.1007/978-1-0716-3782-1_1.  2024.