Research: OLSON and COLLEAGUES

Listed in Issue 244

Abstract

OLSON and COLLEAGUES,  (1)DSM Nutritional Products, 6480 Dobbin Road, Columbia, MD, 21045, USA evaluated the anti-inflammatory activity of docosahexaenoic acid (DHA), alone and in combination with eicosapentaenoic acid (EPA) in a murine model of rheumatoid arthritis, collagen induced arthritis (CIA).

Background

Methodology

The CIA was induced in DBA/1OlaHsd mice by the injection of bovine type II collagen in Freunds's complete adjuvant on days 0 and 21. Mice were fed modified diets containing DHA and/or EPA for 4 weeks prior to the initial collagen injection until study termination at day 45. The severity of CIA was assessed by measuring erythema, edema and mobility of the digits on the fore and hind paws, as well as histology. The level of serum anti-collagen antibodies was determined by ELISA. The ex vivo effects of DHA and/or EPA on splenocyte proliferation and cytokine production were evaluated by BrdU method and ELISA.

Results

Prophylactic treatment with DHA, and not DHA/EPA, significantly reduced arthritis severity and joint damage. Treatment with DHA also decreased anti-collagen (CII) antibodies in vivo, down regulated interleukin-1β, interferonγ and upregulated protective interleukin-10 ex vivo.

Conclusion

Prophylactic treatment with DHA was efficacious in a mouse model of rheumatoid arthritis and may be a useful intervention strategy against inflammatory arthritis.

References

Olson MV(1), Liu YC, Dangi B, Paul Zimmer J, Salem N Jr, Nauroth JM. Docosahexaenoic acid reduces inflammation and joint destruction in mice with collagen-induced arthritis. Inflamm Res. 62(12):1003-13. doi: 10.1007/s00011-013-0658-4. Dec 2013. Epub Sep 6 2013.

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