Positive Health Online

Abstract

LEWANDOWSKA and COLLEAGUES, (1)Department of Biomolecular Chemistry, Medical University of Lodz, Lodz, Poland; (2)Department of Medical Enzymology, Medical University of Lodz, Lodz, Poland; (3)Institute of Technical Biochemistry, Technical University of Lodz, Lodz, Poland studied the effect of an evening primrose extract (EPE)  upon viability and invasiveness of three human cell lines: PNT1A (normal prostate cells), DU145 (prostate cancer cells) and MDA-MB-231 (breast cancer cells).

Background

There is growing interest in plant polyphenols which exhibit pleiotropic biological activities, including anti-inflammatory, antioxidant, and anticancer effects.

Methodology

The authors evaluated the influence of an evening primrose extract (EPE) from defatted seeds on viability and invasiveness of three human cell lines: PNT1A (normal prostate cells), DU145 (prostate cancer cells) and MDA-MB-231 (breast cancer cells).

Results

The results revealed that after 72 h of incubation the tested extract reduced the viability of DU 145 and MDA-MB-231 with IC50 equal to 14.5 μg/mL for both cell lines. In contrast, EPE did not inhibit the viability of normal prostate cells. Furthermore, EPE reduced PNT1A and MDA-MB-231 cell invasiveness; at the concentration of 21.75 μg/mL the suppression of invasion reached 92% and 47%, respectively (versus control). Additionally, zymographic analysis revealed that after 48 h of incubation EPE inhibited metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9) activities in a dose-dependent manner. For PNT1A the activities of MMP-2 and MMP-9 decreased 4- and 2-fold, respectively, at EPE concentration of 29 μg/mL. In the case of MDA-MB-231 and DU 145 the decrease in MMP-9 activity at EPE concentration of 29 μg/mL was 5.5-fold and almost 1.9-fold, respectively.

Conclusion

In conclusion, this study suggests that EPE may exhibit anti-migratory, anti-invasive and antimetastatic potential towards prostate and breast cancer cell lines.

References

Lewandowska U(1), Owczarek K(1), Szewczyk K(2), Podsędek A(3), Koziołkiewicz M(3), Hrabec E(2). Influence of polyphenol extract from evening primrose (Oenothera paradoxa) seeds  on human prostate and breast cancer cell lines. Postepy Hig Med Dosw (Online). 68:110-8. doi: 10.5604/17322693.1088036. Feb 3 2014.

Comment

The above antimetastatic and anti-invasive effects of evening primrose extract (EPE) in prostate and breast cancer cells is encouraging and could help in the development of clinical treatment options.