Positive Health Online

Abstract

GAO and COLLEAGUES, 1 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. 13720161459@163.com ;             2 Institute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan, Hubei 430070, China. wgzhau@163.com ; 3 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. HuangZhiCheng1210@163.com ; 4 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. xiaoyulei1988@126.com ; 5 Institute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan, Hubei 430070, China. bianyinbing@mail.hzau.edu.cn ; 6 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. yingliu@mail.hzau.edu.cn ; 7 College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. huangwen@mail.hzau.edu.cn conducted a study and assessed the expression profile of 17 candidate genes in L. edodes under treatment.

Background

Volatile sulphur compounds gradually develop in Lentinula edodes after hot-air drying, and many genes are involved in the generation of these sulphur compounds. The expression stability of reference genes may vary in a particular experimental treatment when analyzing their expressions by quantitative real-time polymerase chain reaction (qRT-PCR).

Methodology

In this study, the expression profile of 17 candidate genes was assessed in L. edodes under treatment at 50 °C for 0, 1, 2, and 3 h, and the expression stability of each reference gene was analyzed by three statistical algorithms, including geNorm, NormFinder, and BestKeeper.

Results

Results indicated that the two optimal reference genes for mycelium and fruiting body were CAC and DAHP as well as CAC and NUP, respectively. Additionally, CAC and DAHP were found to be the two most stable reference genes across the mycelium and fruiting body set.

Conclusion

Our results will provide a genetic foundation for further research on the metabolism genes of sulfur compounds in L. edodes.  Conflict of interest statement  The authors declare that they have no conflict of interest.

References

Shuangshuang Gao  1 , Gangzheng Wang  2 , Zhicheng Huang  3 , Xiaoyu Lei  4 , Yinbing Bian  5 , Ying Liu  6 , Wen Huang  7  Selection of Reference Genes for qRT-PCR Analysis in Lentinula edodes after Hot-Air Drying  Molecules.  ;24(1):136. doi: 10.3390/molecules24010136.  Dec 31 2018.