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Research: CEBECIOGLU and COLLEAGUES,
Listed in Issue 284
Abstract
CEBECIOGLU and COLLEAGUES, Koc University - Biomedical Sciences and Engineering, Turkey, examined the effects of quercetin and selenium on oxidative stress caused by both hydrogen peroxide and UV radiation in endometrial adenocarcinoma cells.
Background
The effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells are unclear. In this study, the effects of quercetin and selenium on oxidative stress caused by both hydrogen peroxide and UV radiation in endometrial adenocarcinoma cells were examined.
Methodology
The viability of endometrial adenocarcinoma cells cultured in vitro and treated with different concentrations of quercetin and sodium selenite was measured using the MTT assay. Malondialdehyde (MDA) levels were investigated, and expression levels of BAD and p53 genes were analysed using real time quantitative polymerase chain reaction. Acridine orange/ethidium bromide staining technique was applied to detect apoptosis. Mass attenuation coefficient of each quercetin and sodium selenite combinations was evaluated using Monte Carlo simulation.
Results
The combination of quercetin and sodium selenite enhanced cell viability, and reduced MDA levels. The expression levels of BAD and p53 genes decreased by combined treatment with quercetin and selenium while showing synergistic effects in terms of gene expression. Fluorescent microscopic examination showed a decrease in apoptotic cells in endometrial adenocarcinoma cells treated with the combination of quercetin and selenium.
Conclusion
For the first time, selenium and quercetin have synergistic cytoprotective and radioprotective effects on oxidative stress caused by hydrogen peroxide in endometrial adenocarcinoma cells for the first time (Tab. 1, Fig. 7, Ref. 39).
References
R Cebecioglu, M Yildirim, D Akagunduz, I Korkmaz, H O Tekin, B Atasever-Arslan, T Catal. Synergistic effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells; Bratisl Lek Listy;120(6) :449-455. doi: 10.4149/BLL_2019_072. 2019.
